COM_MIC_TEXT_TITLE

*本演講須事先以E-mail報名

請將個人基本資料寄至本中心公務信箱 This email address is being protected from spambots. You need JavaScript enabled to view it.

以該公務信箱所回覆確認信函為憑 30人 為限

*Reservation Confirmation E-mail Required 

Abstract

Based on third-harmonic-generation (THG) microscopy and a k-means clustering algorithm, we developed a label-free imaging cytometry method to differentiate and determine the types of human leukocytes. According to the size and average intensity of cells in THG images, in a two-dimensional scatter plot, the neutrophils, monocytes, and lymphocytes in peripheral blood samples from healthy volunteers were clustered into three differentiable groups. Using these features in THG images, we could count the number of each of the three leukocyte types both in vitro and in vivo. The THG imaging-based counting results agreed well with conventional blood count results. In the future, we believe that the combination of this THG microscopy-based imaging cytometry approach with advanced texture analysis of sub-cellular features can differentiate and count more types of blood cells with smaller quantities of blood.